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Antibodies bound to the blocking peptide not bind for the epitope within the goal protein. By evaluating the staining from the blocked antibody vs . the antibody by itself, you can see which staining is specific.

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This guide outlines even further feasible optimization of cellular and immunoassay parameters to be sure the very best effects are...

This guideline outlines further feasible optimization of cellular and immunoassay parameters to guarantee the absolute best final results are...

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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the images or links will redirect you to a web site hosted by BenchSci that provides third-party scientific material. Neither the content nor the BenchSci know-how and procedures for range have been evaluated by us; we have been delivering them as-is and devoid of guarantee of any variety, together with to be used or software from the Thermo Fisher Scientific goods introduced.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide normally has the epitope recognized by the antibody.

Target Info Cyclin E belongs towards the extremely conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic function. Cyclin E forms a fancy with and capabilities for read more a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.

Overexpression of the gene continues to be noticed in many tumors, which ends up in chromosome instability, and so may perhaps contribute to tumorigenesis. This protein was uncovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical role in promoting mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but specific nucleotide sequence info just isn't nonetheless obtainable.

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